Histone H4 (tri methyl K20) (2C12) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Histone H4 (tri methyl K20) (2C12) Monoclonal Antibody (bsm-52112R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.\r\n \r\n$

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Histone H4 (tri methyl K20) (2C12) Monoclonal Antibody (bsm-52112R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.\r\n \r\n

Applications

IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52112R
Product Name	Histone H4 (tri methyl K20) (2C12) Monoclonal Antibody
Applications	IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide derived from human Histone H4 (tri methyl K20), around 1-30aa (tri methyl K20).
Modification Site	tri methyl K20
Clonality	Monoclonal
Clone #	2C12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.
Target
Gene ID	121504
Swiss Prot	P62805
Synonyms	Histone H4 Acetyl K12; Histone H4 Acetyl Lys12; Acetyl-Histone H4 Lys12; H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN.
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].
Application Dilution
IHC-P	1:200-400

Applications

Reactivity

Search our catalog of hundreds of thousands of products