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IRF1 Recombinant Antibody

HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with IRF1 (3G7) Monoclonal Antibody (bsm-52114R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with IRF1 (3G7) Monoclonal Antibody (bsm-52114R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-52114r
Product Name IRF1 Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human IRF1 protein, around C-terminal 100aa
Clonality Recombinant
Clone # 3G7
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3659
Swiss Prot P10914
Synonyms interferon regulatory factor 1; IRF 1; IRF1; MAR1; IRF1_HUMAN.
Application Dilution
WB 1:300-5000
IHC-P FCM1:20-100
IHC-F IHC-P1:200-400
IF IHC-P1:50-200
Flow-Cyt IFICC1:50-200
ICC/IF

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