JNK2 (1C7) Monoclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52121r-pe-cy5-100ul |
| Product Name | JNK2 (1C7) Monoclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | Recombinant human JNK2 protein, around C-terminal 150aa. |
| Clonality | Monoclonal |
| Clone # | 1C7 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5601 |
| Swiss Prot | P45984 |
| Synonyms | JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA; Mitogen-activated protein kinase 9; MAP kinase 9; MAPK 9; Stress-activated protein kinase 1a; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; MAPK9 |
| Background | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is JNK2 (1C7) Monoclonal Antibody, PE-Cy5 Conjugated (bsm-52121R-PE-Cy5) validated for, and at what dilution?
bsm-52121R-PE-Cy5 is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does JNK2 (1C7) Monoclonal Antibody, PE-Cy5 Conjugated (bsm-52121R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52121R-PE-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise JNK2 (1C7) Monoclonal Antibody, PE-Cy5 Conjugated (bsm-52121R-PE-Cy5)?
Recombinant human JNK2 protein, around C-terminal 150aa.
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bsm-52121R-PE-Cy5)?
PE-Cy5 on bsm-52121R-PE-Cy5: excitation / emission 488nm/670nm.