Free Shipping On Orders Over $1,000!

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

AKT1 Monoclonal Antibody

Lane 1: NIH\/3T3 treated with PDGF; Lane 2: NIH\/3T3 untreatedlysates probed with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody. The bottom panel is antibody staining using an unphosphorylated AKT1 antibody.
Lane 1: NIH\/3T3 treated with PDGF; Lane 2: NIH\/3T3 untreatedlysates probed with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody. The bottom panel is antibody staining using an unphosphorylated AKT1 antibody.
3T3 cells were stained with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
3T3 cells were stained with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse lung section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse lung section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
MCF7 cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Akt1 (Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Akt1 (Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Akt1(Ser473) (12A1) Monoclonal Antibody, Unconjugated (bsm-52130R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Akt1(Ser473) (12A1) Monoclonal Antibody, Unconjugated (bsm-52130R) at 1:200 overnight at 4\u00b0C, DAB staining.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Akt1(Ser473) (12A1) Monoclonal Antibody(bsm-52130R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Akt1(Ser473) (12A1) Monoclonal Antibody(bsm-52130R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Akt1(Ser473) (12A1) Monoclonal Antibody, Unconjugated (bsm-52130R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Akt1(Ser473) (12A1) Monoclonal Antibody, Unconjugated (bsm-52130R) at 1:200 overnight at 4\u00b0C, DAB staining.
Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): Jurkat, (+): Jurkat+ Calyculin A (100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 56 kDa Observed MW: 56, 60 kDa
Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): Jurkat, (+): Jurkat+ Calyculin A (100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 56 kDa Observed MW: 56, 60 kDa
Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate:(-): MCF-7, (+): MCF-7+ Calyculin A (100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 56 kDa Observed MW: 56, 60 kDa
Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate:(-): MCF-7, (+): MCF-7+ Calyculin A (100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 56 kDa Observed MW: 56, 60 kDa
Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate:(-): HeLa, (+): HeLa+ Calyculin A (100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 56 kDa Observed MW: 56, 60 kDa
Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate:(-): HeLa, (+): HeLa+ Calyculin A (100nM, 30min) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 56 kDa Observed MW: 56, 60 kDa
Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate:(-): NIH\/3T3, (+): NIH\/3T3+ Calyculin A (100ng\/ml, 60min) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 56 kDa Observed MW: 56, 60 kDa
Blocking buffer: 5% NFDM\/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate:(-): NIH\/3T3, (+): NIH\/3T3+ Calyculin A (100ng\/ml, 60min) Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 56 kDa Observed MW: 56, 60 kDa
Lane 1: Human Jurkat cell lysates; Lane 2: Human MOLT4 cell lysates probed with Phospho-Akt1(Ser473) Monoclonal Antibody, Unconjugated (bsm-52130R) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Jurkat cell lysates; Lane 2: Human MOLT4 cell lysates probed with Phospho-Akt1(Ser473) Monoclonal Antibody, Unconjugated (bsm-52130R) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • FCM
  • IHC-P
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52130R
Product Name AKT1 Monoclonal Antibody
Applications WB, FCM, IHC-P, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473:QF(p-S)YS.
Modification Site Ser473
Clonality Monoclonal
Clone # 12A1
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 207
Swiss Prot P31749
Synonyms AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; PKBG
Background AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

Search our catalog of hundreds of thousands of products