phospho-Akt (Thr450) Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52131r-20uL |
| Product Name | phospho-Akt (Thr450) Recombinant Antibody |
| Applications |
IHC-P
IHC-F
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide derived from human AKT1(T450), around 400-470aa (phospho T450). |
| Modification Site | T450 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 207 |
| Swiss Prot | P31749 |
| Synonyms | AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; PKBG |
| Background | AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. |
| Application Dilution | |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF | |
Frequently asked questions
Which applications is phospho-Akt (Thr450) Recombinant Antibody (bsm-52131R) validated for, and at what dilution?
bsm-52131R is validated for IHC-P (1:200-400); IHC-F (1:100-500); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Akt (Thr450) Recombinant Antibody (bsm-52131R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52131R be stored?
Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise phospho-Akt (Thr450) Recombinant Antibody (bsm-52131R)?
Synthetic peptide derived from human AKT1(T450), around 400-470aa (phospho T450).