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eIF4E (Ser209) Recombinant Antibody

Lane 1: Mouse Pancreas tissue lysates; Lane 2:Rat Lymph node tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Pancreas tissue lysates ; Lane 5: Human Hela cell lysates;Lane 6: Human 293T cell lysates ;Lane 7: Human MCF-7 cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with eIF4E(S209) (2B1) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Pancreas tissue lysates; Lane 2:Rat Lymph node tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Pancreas tissue lysates ; Lane 5: Human Hela cell lysates;Lane 6: Human 293T cell lysates ;Lane 7: Human MCF-7 cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with eIF4E(S209) (2B1) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52152r-100uL
Product Name eIF4E (Ser209) Recombinant Antibody
Applications
WB IHC-F IF IHC-P ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209
Modification Site S209
Clonality Recombinant
Clone # 2B1
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1977
Swiss Prot P06730
Subcellular location Cytoplasm, Nucleus
Synonyms AUTS19; CBP; EIF4E1; EIF4EL1; EIF4F; eIF-4E; EG668879; Eif4e-ps; If4e; IF4E_HUMAN; EIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; IF4E_MOUSE; IF4E_RAT; eukaryotic translation initiation factor 4E; eIF4E (phospho-S209); p-eIF4E; phospho-eIF4E; eIF4E (phospho-Ser209)
Background Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Application Dilution
WB 1:500-2000
IHC-F 1:100-500
IF 1:100-500
IHC-P 1:100-500
ICC/IF 1:50-200

Frequently asked questions

Which applications is eIF4E (Ser209) Recombinant Antibody (bsm-52152R) validated for, and at what dilution?

bsm-52152R is validated for WB (1:500-2000); IHC-F (1:100-500); IF (1:100-500); IHC-P (1:100-500); ICC/IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does eIF4E (Ser209) Recombinant Antibody (bsm-52152R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-52152R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise eIF4E (Ser209) Recombinant Antibody (bsm-52152R)?

KLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209

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