Phospho-Glycogen synthase 1 (Ser641) Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52159r-cy5-100uL |
| Product Name | Phospho-Glycogen synthase 1 (Ser641) Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | Synthetic peptide derived from human Glycogen synthase 1(S641), around 630-680aa (phospho S641). |
| Modification Site | S641 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2997 |
| Swiss Prot | P13807 |
| Synonyms | Glycogen synthase 1 phospho S640; Glycogen synthase 1 phospho Ser640; p-Glycogen synthase 1 S640; Glycogen synthase 1 muscle; Glycogen synthase 1; GSY; GYS; GYS1; EC 2.4.1.11; Glycogen synthase1; GYS 1; Starchsynthase muscle; UDP glucose glycogen glucosyltransferase; GYS1_HUMAN; Glycogen [starch] synthase, muscl. |
| Background | Glycogen Synthase (GS) is a key enzyme in the regulation of glycogen metabolism. GS catalyzes the incorporation of UDP-glucose incorporation into glycogen. The activity of glycogen synthase is regulated by hormonal stimuli (insulin, catecholamines and glucagons) and non-hormonal stimuli (blood glucose level and exercise). Two main isoforms of mammalian GS are designated as muscle (glycogen synthase 1) and liver (glycogen synthase 2). Most tissues express glycogen synthase 1, whereas glycogen synthase 2 appears to be tissue-specific. The two isoforms have 70% identical amino acid sequence. Glycogen synthase can be phosphorylated by multiple kinases including glycogen synthase kinase-3 (GSK-3), mitogen-activated protein kinase-related protein kinase (DYRK), and SAPK2b/p38b which leads to its inactivation. |
| Application Dilution | |
| WB | 1:20-50 |
| IF | 1:100-500 |
| Flow-Cyt | 1:500-2000 |
Frequently asked questions
Which applications is Phospho-Glycogen synthase 1 (Ser641) Recombinant Antibody, Cy5 Conjugated (bsm-52159R-Cy5) validated for, and at what dilution?
bsm-52159R-Cy5 is validated for WB (1:20-50); IF (1:100-500); Flow-Cyt (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Glycogen synthase 1 (Ser641) Recombinant Antibody, Cy5 Conjugated (bsm-52159R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52159R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Glycogen synthase 1 (Ser641) Recombinant Antibody, Cy5 Conjugated (bsm-52159R-Cy5)?
Synthetic peptide derived from human Glycogen synthase 1(S641), around 630-680aa (phospho S641).
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-52159R-Cy5)?
Cy5 on bsm-52159R-Cy5: excitation / emission 625,650nm/670nm.