Phospho-H2AX (Ser139) Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52163r-bf647-100uL |
| Product Name | Phospho-H2AX (Ser139) Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Ser139:QA(p-S)QE. |
| Modification Site | S139 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3014 |
| Swiss Prot | P16104 |
| Synonyms | H2A histone family member X; Histone H2A.x; H2afx; H2a.x; H2ax; Hist5-2ax; H2A.X; H2A/X; H2AX; H2AFX; H2AX_HUMAN. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Phospho-H2AX (Ser139) Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52163R-BF647) validated for, and at what dilution?
bsm-52163R-BF647 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-H2AX (Ser139) Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52163R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52163R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-H2AX (Ser139) Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52163R-BF647)?
KLH conjugated Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Ser139:QA(p-S)QE.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-52163R-BF647)?
AbBy Fluor-647 on bsm-52163R-BF647: excitation / emission 650nm/665nm.