phospho-HSF1 (Ser326) Recombinant Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52166r-apc-cy7-100uL |
| Product Name | phospho-HSF1 (Ser326) Recombinant Antibody, APC-Cy7 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | Synthetic peptide derived from human HSF1(S326), around 300-350aa (phospho S326). |
| Modification Site | S326 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3297 |
| Swiss Prot | Q00613 |
| Synonyms | HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1; HSF1 |
| Background | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is phospho-HSF1 (Ser326) Recombinant Antibody, APC-Cy7 Conjugated (bsm-52166R-APC-Cy7) validated for, and at what dilution?
bsm-52166R-APC-Cy7 is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-HSF1 (Ser326) Recombinant Antibody, APC-Cy7 Conjugated (bsm-52166R-APC-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bsm-52166R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HSF1 (Ser326) Recombinant Antibody, APC-Cy7 Conjugated (bsm-52166R-APC-Cy7)?
Synthetic peptide derived from human HSF1(S326), around 300-350aa (phospho S326).
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bsm-52166R-APC-Cy7)?
APC-Cy7 on bsm-52166R-APC-Cy7: excitation / emission 650nm/780nm.