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PLCG1 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with PLCG1 monoclonal antibody, unconjugated (bsm-52174R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PLCG1 monoclonal antibody, unconjugated (bsm-52174R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PLCG1 antibody (bsm-52174R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PLCG1 antibody (bsm-52174R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-52174r-100uL
Product Name PLCG1 Recombinant Antibody
Applications
WB IHC-P IHC-F IF ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human Phospholipase C gamma 1 protein, around C-terminal 150aa.
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 5335
Swiss Prot P19174
Synonyms PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C-gamma-1; PLC-gamma-1; PLCG1
Background Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF
ICC/IF 1:50-100

Frequently asked questions

Which applications is PLCG1 Recombinant Antibody (bsm-52174R) validated for, and at what dilution?

bsm-52174R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF; ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PLCG1 Recombinant Antibody (bsm-52174R) react with?

Tested reactivity: Human.

How should bsm-52174R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise PLCG1 Recombinant Antibody (bsm-52174R)?

Recombinant human Phospholipase C gamma 1 protein, around C-terminal 150aa.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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