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Phospho-PKR (Thr446) Recombinant Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-52189r-bf750
Product Name Phospho-PKR (Thr446) Recombinant Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF
Reactivity Human
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source A synthesized peptide derived from human eIF2A K2 around the phosphorylation site of T446
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5610
Swiss Prot P19525
Subcellular location Cytoplasm, Nucleus
Synonyms PKR(T446); PKR(phospho T446); p-PKR(phospho T446); PKR(phospho-Thr446); double-stranded RNA-dependent Protein Kinase; interferon-induced, double-stranded RNA-activated protein kinase isoform a; protein kinase, interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; protein kinase RNA-activated; interferon-inducible RNA-dependent protein kinase; EIF2AK2; EIF2AK1; MGC126524; PKR; PRKRv; E2AK2_HUMAN.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is Phospho-PKR (Thr446) Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-52189R-BF750) validated for, and at what dilution?

bsm-52189R-BF750 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-PKR (Thr446) Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-52189R-BF750) react with?

Tested reactivity: Human.

How should bsm-52189R-BF750 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-PKR (Thr446) Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-52189R-BF750)?

A synthesized peptide derived from human eIF2A K2 around the phosphorylation site of T446

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bsm-52189R-BF750)?

AbBy Fluor-750 on bsm-52189R-BF750: excitation / emission 749nm/775nm.