RSK1 p90(T359+S363) (8H2) Monoclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52198r-bf594-100ul |
| Product Name | RSK1 p90(T359+S363) (8H2) Monoclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | Synthetic peptide derived from human RSK1 p90(T359+S363), around 300-400aa (phospho T359+S363). |
| Modification Site | T359+S363 |
| Clonality | Monoclonal |
| Clone # | 8H2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6195 |
| Swiss Prot | Q15418 |
| Synonyms | p90 RSK1; p90rsk; p90S6K; pp90RSK1; Ribosomal protein S6 kinase 90kD 1; Ribosomal protein S6 kinase 90kD polypeptide 1; Ribosomal protein S6 kinase 90kDa polypeptide 1; Ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase polypeptide 1; Ribosomal S6 kinase 1; RPS6K1 alpha; rps6ka; Rps6ka1; RSK 1; RSK 1 p90; RSK; RSK1; S6K alpha 1; 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 ribosomal protein S6 kinase, 90kD, polypeptide 1; dJ590P13.1; EC 2.7.11.1; HU 1; HU1; MAP kinase activated protein kinase 1a; MAPKAP kinase 1A; MAPKAPK1A; MGC79981; Mitogen-activated protein kinase-activated protein kinase 1A |
| Background | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). |
| Application Dilution | |
| WB | 1:1000-2000 |
| IF | 1:50-200 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is RSK1 p90(T359+S363) (8H2) Monoclonal Antibody, AbBy Fluor-594 Conjugated (bsm-52198R-BF594) validated for, and at what dilution?
bsm-52198R-BF594 is validated for WB (1:1000-2000); IF (1:50-200); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RSK1 p90(T359+S363) (8H2) Monoclonal Antibody, AbBy Fluor-594 Conjugated (bsm-52198R-BF594) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-52198R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RSK1 p90(T359+S363) (8H2) Monoclonal Antibody, AbBy Fluor-594 Conjugated (bsm-52198R-BF594)?
Synthetic peptide derived from human RSK1 p90(T359+S363), around 300-400aa (phospho T359+S363).
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-52198R-BF594)?
AbBy Fluor-594 on bsm-52198R-BF594: excitation / emission 590nm/617nm.