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phospho-SIRT1 (Thr530) Recombinant Antibody

HepG2 cells were stained with SIRT1(T530) (6H5) Monoclonal Antibody (bsm-52202R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HepG2 cells were stained with SIRT1(T530) (6H5) Monoclonal Antibody (bsm-52202R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells were stained with SIRT1(T530) (6H5) Monoclonal Antibody (bsm-52202R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells were stained with SIRT1(T530) (6H5) Monoclonal Antibody (bsm-52202R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-52202r
Product Name phospho-SIRT1 (Thr530) Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF, ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic peptide derived from human SIRT1(T530), around 500-550aa (phospho T530).
Modification Site T530
Clonality Recombinant
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 23411
Swiss Prot Q96EB6
Synonyms SIR2L1; NAD-dependent protein deacetylase sirtuin-1; hSIRT1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2; SIRT1
Background This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF
ICC/IF 1:50-100

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