phospho-SIRT1 (Thr530) Recombinant Antibody

HepG2 cells were stained with SIRT1(T530) (6H5) Monoclonal Antibody (bsm-52202R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

HeLa cells were stained with SIRT1(T530) (6H5) Monoclonal Antibody (bsm-52202R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

$25 ug total protein per lane of various lysates (see on figure) probed with phospho-SIRT1 (Thr530) monoclonal antibody, unconjugated (bsm-52202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with phospho-SIRT1 (Thr530) monoclonal antibody, unconjugated (bsm-52202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-52202r
Product Name	phospho-SIRT1 (Thr530) Recombinant Antibody
Applications	WB IHC-P IHC-F IF ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthesised phosphopeptide derived from human SIRT1 around the phosphorylation site of Thr530
Modification Site	T530
Clonality	Recombinant
Clone #	6H5
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23411
Swiss Prot	Q96EB6
Subcellular location	Cytoplasm, Nucleus
Synonyms	SIR2; SIR2L1; SIR2alpha; Sir2a; SIR1_HUMAN; SIRT1; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1 (hSIR2); 2.3.1.286; SIR1_MOUSE; SIR2-like protein 1; SIR2alpha (Sir2 \| mSIR2a); sirtuin 1; sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); SIRT1 (phospho-T530); p-SIRT1; phospho-SIRT1; SIRT1 (phospho-Thr530)
Background	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC/IF	1:50-100