phospho-Smad2 (Ser255) Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52204r |
| Product Name | phospho-Smad2 (Ser255) Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide derived from human Smad2(S255), around 220-280aa (phospho S255). |
| Modification Site | S255 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 4087 |
| Swiss Prot | Q15796 |
| Synonyms | JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; Mad-related protein 2; SMAD family member 2; SMAD 2; Smad2 |
| Background | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Application Dilution | |
| WB | 1:1000IHC1:50-200 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF | |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is phospho-Smad2 (Ser255) Recombinant Antibody (bsm-52204R) validated for, and at what dilution?
bsm-52204R is validated for WB(WB(1:1000)IHC) (1:50-200); IHC-P (1:200-400); IHC-F (1:100-500); IF; ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Smad2 (Ser255) Recombinant Antibody (bsm-52204R) react with?
Tested reactivity: Human, Mouse.
How should bsm-52204R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise phospho-Smad2 (Ser255) Recombinant Antibody (bsm-52204R)?
Synthetic peptide derived from human Smad2(S255), around 220-280aa (phospho S255).