Phospho-Smad3 (Ser423 + Ser425) Recombinant Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52205r-100uL |
| Product Name | Phospho-Smad3 (Ser423 + Ser425) Recombinant Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4088 |
| Swiss Prot | P84022 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Smad3(phospho Ser423/425); Smad3(phospho S423+S425); p-Smad3(Ser423+Ser425); hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD(mothers against decapentaplegic Drosophila) homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-Smad3 (Ser423 + Ser425) Recombinant Antibody (bsm-52205R) validated for, and at what dilution?
bsm-52205R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Smad3 (Ser423 + Ser425) Recombinant Antibody (bsm-52205R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-52205R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Smad3 (Ser423 + Ser425) Recombinant Antibody (bsm-52205R)?
KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425
