phospho-SMAD5 (Ser463 + Ser465) Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52206r-cy7-100uL |
| Product Name | phospho-SMAD5 (Ser463 + Ser465) Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465:IS(p-S)V(p-S). |
| Modification Site | S463/465 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4090 |
| Swiss Prot | Q99717 |
| Synonyms | DWFC; JV5-1; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; SMAD family member 5; SMAD 5; Smad5; hSmad5 |
| Background | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is phospho-SMAD5 (Ser463 + Ser465) Recombinant Antibody, Cy7 Conjugated (bsm-52206R-Cy7) validated for, and at what dilution?
bsm-52206R-Cy7 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-SMAD5 (Ser463 + Ser465) Recombinant Antibody, Cy7 Conjugated (bsm-52206R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52206R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-SMAD5 (Ser463 + Ser465) Recombinant Antibody, Cy7 Conjugated (bsm-52206R-Cy7)?
KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465:IS(p-S)V(p-S).
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-52206R-Cy7)?
Cy7 on bsm-52206R-Cy7: excitation / emission 743nm/767nm.