Phospho-YAP1 (Ser127) Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52214r-rbitc |
| Product Name | Phospho-YAP1 (Ser127) Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | A synthesized peptide derived from human YAP1 around the phosphorylation site of S127 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10413 |
| Swiss Prot | P46937 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | phospho-YAP1(S127); YAP1(S127); YAP1(phospho Ser127); YAP1(phospho S127); 65 kDa Yes associated protein; YAp 1; YAP 65; YAP; YAP2; YAP65; Yes associated protein 1 65kDa; Yes associated protein 1; Yes associated protein 2; YKI; Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog; YAP1_HUMAN. |
| Application Dilution | |
| WB | 1:500-5000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is Phospho-YAP1 (Ser127) Recombinant Antibody, RBITC Conjugated (bsm-52214R-RBITC) validated for, and at what dilution?
bsm-52214R-RBITC is validated for WB(WB) (1:500-5000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-YAP1 (Ser127) Recombinant Antibody, RBITC Conjugated (bsm-52214R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52214R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-YAP1 (Ser127) Recombinant Antibody, RBITC Conjugated (bsm-52214R-RBITC)?
A synthesized peptide derived from human YAP1 around the phosphorylation site of S127
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-52214R-RBITC)?
RBITC on bsm-52214R-RBITC: excitation / emission 570nm/595nm.