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PI 3 Kinase p85 alpha (2G4) Monoclonal Antibody

Lane 1: MCF-7; Lane 2: Raji lysate probed with PI 3 Kinase p85 alpha (2G4) Monoclonal Antibody (bsm-52216R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody .
Lane 1: MCF-7; Lane 2: Raji lysate probed with PI 3 Kinase p85 alpha (2G4) Monoclonal Antibody (bsm-52216R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody .
HeLa cells were stained with PI 3 Kinase p85 alpha (2G4) Monoclonal Antibody (bsm-52216R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells were stained with PI 3 Kinase p85 alpha (2G4) Monoclonal Antibody (bsm-52216R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Spleen lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human K562 cell lysates; Lane 7: Human MOLT-4 cell lysates; Lane 8: Human HL-60 cell lysates probed with PI 3 Kinase p85 alpha Monoclonal Antibody, Unconjugated (bsm-52216R ) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Spleen lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human K562 cell lysates; Lane 7: Human MOLT-4 cell lysates; Lane 8: Human HL-60 cell lysates probed with PI 3 Kinase p85 alpha Monoclonal Antibody, Unconjugated (bsm-52216R ) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52216R
Product Name PI 3 Kinase p85 alpha (2G4) Monoclonal Antibody
Applications WB, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human PI 3 Kinase p85 alpha protein, around 600-700aa.
Clonality Monoclonal
Clone # 2G4
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months..
Target
Gene ID 5295
Swiss Prot P27986
Synonyms p85; AGM7; GRB1; IMD36; p85-ALPHA; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1
Background Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.
Application Dilution
WB 1:300-1000
IF(ICC) 1:50-200

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