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Smad5 (7A1) Monoclonal Antibody

Lane 1: PC-3 lysates probed with Smad5 (7A1) Monoclonal Antibody (bsm-52226R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.
Lane 1: PC-3 lysates probed with Smad5 (7A1) Monoclonal Antibody (bsm-52226R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.
A549 cells were stained with Smad5 (7A1) Monoclonal Antibody (bsm-52226R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
A549 cells were stained with Smad5 (7A1) Monoclonal Antibody (bsm-52226R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52226R
Product Name Smad5 (7A1) Monoclonal Antibody
Applications WB, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human Smad5 protein, around 200-300aa.
Clonality Monoclonal
Clone # 7A1
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months..
Target
Gene ID 4090
Swiss Prot Q99717
Synonyms DWFC; JV5-1; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; SMAD family member 5; SMAD 5; Smad5; hSmad5
Background Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Application Dilution
WB 1:300-1000
IF(ICC) 1:50-200

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