STAT2 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with STAT2 monoclonal antibody, unconjugated (bsm-52234R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with STAT2 monoclonal antibody, unconjugated (bsm-52234R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ICC/IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52234r
Product Name	STAT2 Recombinant Antibody
Applications	WB ICC/IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human STAT2 protein, around N-terminal 1-100aa.
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	6773
Swiss Prot	P52630
Synonyms	P113; ISGF-3; STAT113; Signal transducer and activator of transcription 2; STAT2
Background	Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Application Dilution
WB	1:300-5000
ICC/IF	1:50-100