Stat3 Recombinant Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52235r-apc-Cy5.5-100uL |
| Product Name | Stat3 Recombinant Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | Recombinant human Stat3 protein, around N-terminal 1-100aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6774 |
| Swiss Prot | P40763 |
| Synonyms | APRF; HIES; ADMIO; Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3 |
| Background | Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is Stat3 Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-52235R-APC-CY5-5) validated for, and at what dilution?
BSM-52235R-APC-CY5-5 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Stat3 Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-52235R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-52235R-APC-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Stat3 Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-52235R-APC-CY5-5)?
Recombinant human Stat3 protein, around N-terminal 1-100aa.
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BSM-52235R-APC-CY5-5)?
APC-Cy5.5 on BSM-52235R-APC-CY5-5: excitation / emission 650nm/694nm.