STAT4 (4E5) Monoclonal Antibody

$Daudi cell lysate probed with STAT4 (4E5) Monoclonal Antibody, Unconjugated (bsm-52236R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.$

Daudi cell lysate probed with STAT4 (4E5) Monoclonal Antibody, Unconjugated (bsm-52236R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.

$Paraformaldehyde-fixed, paraffin embedded human breast \r\ncarcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with STAT4 (4E5) Monoclonal Antibody (bsm-52236R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human breast \r\ncarcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with STAT4 (4E5) Monoclonal Antibody (bsm-52236R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$MCF-7 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT4 (4E5) Monoclonal Antibody (bsm-52236R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.$

MCF-7 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT4 (4E5) Monoclonal Antibody (bsm-52236R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52236R
Product Name	STAT4 (4E5) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human STAT4 protein, around C-terminal 100aa.
Clonality	Monoclonal
Clone #	400000
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.
Target
Gene ID	6775
Swiss Prot	Q14765
Synonyms	Signal transducer and activator of transcription 4; STAT4
Background	Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
Application Dilution
WB	1:300-1000
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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