Histone H3 (mono methyl K18) Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52244r-apc |
| Product Name | Histone H3 (mono methyl K18) Recombinant Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | Synthetic peptide surrounding K18 of Histone H3 |
| Modification Site | mono methyl K18 |
| Clonality | Monoclonal |
| Clone # | 2H4 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P68431 |
| Synonyms | Histone H3, Histone H3 |
| Background | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Histone H3 (mono methyl K18) Recombinant Antibody, APC Conjugated (bsm-52244R-APC) validated for, and at what dilution?
bsm-52244R-APC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (mono methyl K18) Recombinant Antibody, APC Conjugated (bsm-52244R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52244R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (mono methyl K18) Recombinant Antibody, APC Conjugated (bsm-52244R-APC)?
Synthetic peptide surrounding K18 of Histone H3
What are the excitation and emission wavelengths of the APC conjugate (bsm-52244R-APC)?
APC on bsm-52244R-APC: excitation / emission 650nm/660nm.