NGFR/p75NTR Recombinant Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52253r-hrp-100uL |
| Product Name | NGFR/p75NTR Recombinant Antibody, HRP Conjugated |
| Applications |
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | A synthesized peptide derived from human p75 NTR : 350-400 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4804 |
| Swiss Prot | P08138 |
| Synonyms | Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, p75 ICD, CD_antigen: CD271, NGF receptor, NGFR |
| Background | The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. NGF is important for the development, differentiation and survival of a variety of neuronal and non-neuronal cells. Its action is mediated by binding to two distinct receptors, the high affinity p140 and low affinity p75. p75NGFR binds neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6. p75NGFR belongs to the TNF-R superfamily and is reported to mediate NGF-induced apoptosis. |
| Application Dilution | |
| IHC-P | 1:100-500 |
| IHC-F | IHC-F1:100-500 |
Frequently asked questions
Which applications is NGFR/p75NTR Recombinant Antibody, HRP Conjugated (bsm-52253R-HRP) validated for, and at what dilution?
bsm-52253R-HRP is validated for IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NGFR/p75NTR Recombinant Antibody, HRP Conjugated (bsm-52253R-HRP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52253R-HRP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NGFR/p75NTR Recombinant Antibody, HRP Conjugated (bsm-52253R-HRP)?
A synthesized peptide derived from human p75 NTR : 350-400