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GFAP Recombinant Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52254r-cy5-100uL
Product Name GFAP Recombinant Antibody, Cy5 Conjugated
Applications
WB FCM IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy5
Host Rabbit
Source Full length protein
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2670
Swiss Prot P14136
Synonyms Glial fibrillary acidic protein, GFAP
Background GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Application Dilution
WB 1:1000-50000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is GFAP Recombinant Antibody, Cy5 Conjugated (bsm-52254R-Cy5) validated for, and at what dilution?

bsm-52254R-Cy5 is validated for WB(WB) (1:1000-50000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GFAP Recombinant Antibody, Cy5 Conjugated (bsm-52254R-Cy5) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-52254R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GFAP Recombinant Antibody, Cy5 Conjugated (bsm-52254R-Cy5)?

Full length protein

What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-52254R-Cy5)?

Cy5 on bsm-52254R-Cy5: excitation / emission 625,650nm/670nm.