MLKL (3G4) Monoclonal Antibody

$Lane 1: HT29; Lane 2: HUVEC; Lane 3: Hela lysate; Probed with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HT29; Lane 2: HUVEC; Lane 3: Hela lysate; Probed with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52256R
Product Name	MLKL (3G4) Monoclonal Antibody
Applications	WB, IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human MLKL between 350 to C-terminus
Immunogen Range	350-471/471
Clonality	Monoclonal
Clone #	3G4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	197259
Swiss Prot	Q8NB16
Subcellular location	Cell membrane
Synonyms	Mixed lineage kinase domain-like protein, hMLKL; MLKL
Background	Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed:29883610).
Application Dilution
WB	1:300-5000
IHC

Applications

Reactivity

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