MLKL Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52256r-bf647-100uL |
| Product Name | MLKL Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | Human MLKL between 350 to C-terminus |
| Immunogen Range | 350-471/471 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 197259 |
| Swiss Prot | Q8NB16 |
| Subcellular location | Cell membrane |
| Synonyms | Mixed lineage kinase domain-like protein, hMLKL; MLKL |
| Background | Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed:29883610). |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
Frequently asked questions
Which applications is MLKL Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52256R-BF647) validated for, and at what dilution?
bsm-52256R-BF647 is validated for WB (1:500-2000); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MLKL Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52256R-BF647) react with?
Tested reactivity: Human.
How should bsm-52256R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MLKL Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52256R-BF647)?
Human MLKL between 350 to C-terminus Immunogen range: 350-471/471.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-52256R-BF647)?
AbBy Fluor-647 on bsm-52256R-BF647: excitation / emission 650nm/665nm.