Integrin beta 1 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52266r-cy5-100uL |
| Product Name | Integrin beta 1 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | Recombinant human Integrin beta 1 between 1 to 250 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3688 |
| Swiss Prot | P05556 |
| Subcellular location | Cell membrane, Extracellular domain |
| Synonyms | Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; VLA-4 subunit beta; CD29; GPIIA; ITGB1 |
| Background | Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is Integrin beta 1 Recombinant Antibody, Cy5 Conjugated (bsm-52266R-Cy5) validated for, and at what dilution?
bsm-52266R-Cy5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Integrin beta 1 Recombinant Antibody, Cy5 Conjugated (bsm-52266R-Cy5) react with?
Tested reactivity: Human.
How should bsm-52266R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Integrin beta 1 Recombinant Antibody, Cy5 Conjugated (bsm-52266R-Cy5)?
Recombinant human Integrin beta 1 between 1 to 250 amino acids
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-52266R-Cy5)?
Cy5 on bsm-52266R-Cy5: excitation / emission 625,650nm/670nm.