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Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4 ) Monoclonal Antibody

Lane 1: Untreated CRC whole cell lysates; Lane 2: CRC cells treated with 1.5\u00b5g\/ml Colcemid for 12 hours whole cell lysates; Probed with Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4 ) Monoclonal Antibody (bsm-52267R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Untreated CRC whole cell lysates; Lane 2: CRC cells treated with 1.5µg/ml Colcemid for 12 hours whole cell lysates; Probed with Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4 ) Monoclonal Antibody (bsm-52267R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Applications

  • WB
  • IHC-P
  • IF(ICC)
  • IHC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52267R
Product Name Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4 ) Monoclonal Antibody
Applications WB, IHC-P, IF(ICC), IHC
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic phospho-peptide around Thr17 of human H1.4
Modification Site T17+Histone H1.4
Clonality Monoclonal
Clone # #REF!
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Swiss Prot P10412
Synonyms Histone H1.3, Histone H1c, Histone H1s-2, Histone H1.4, Histone H1b, Histone H1s-4, HIST1H1D, HIST1H1E
Background Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC

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