NeuN Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52268r-bf555-100uL |
| Product Name | NeuN Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | Human NeuN between 1-100 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 146713 |
| Swiss Prot | A6NFN3 |
| Synonyms | RNA binding protein fox-1 homolog 3, Fox-1 homolog C, RBFOX3 |
| Background | Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is NeuN Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-52268R-BF555) validated for, and at what dilution?
bsm-52268R-BF555 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NeuN Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-52268R-BF555) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52268R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NeuN Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-52268R-BF555)?
Human NeuN between 1-100 amino acids
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-52268R-BF555)?
AbBy Fluor-555 on bsm-52268R-BF555: excitation / emission 553nm/568nm.