Prohibitin (1F10) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52282R-100uL |
| Product Name | Prohibitin (1F10) Monoclonal Antibody |
| Applications |
WB
IHC-F
IF(ICC)
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat, Zebrafish, Fish |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | N terminal Human Prohibitin |
| Clonality | Monoclonal |
| Clone # | 1F10 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5245 |
| Swiss Prot | P35232 |
| Subcellular location | Mitochondrion, Membrane bound |
| Synonyms | Prohibitin; PHB |
| Background | Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80% of the cell lines analyzed. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-F | 1:100-500 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) validated for, and at what dilution?
bsm-52282R is validated for WB (1:300-5000); IHC-F (1:100-500); IF(ICC) (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) react with?
Tested reactivity: Human, Mouse, Rat, Zebrafish, Fish.
How should bsm-52282R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Prohibitin (1F10) Monoclonal Antibody (bsm-52282R)?
N terminal Human Prohibitin