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ARPC5 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with ARPC5 monoclonal antibody, unconjugated (bsm-52302R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with ARPC5 monoclonal antibody, unconjugated (bsm-52302R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
Overview
Catalog # bsm-52302r-100uL
Product Name ARPC5 Recombinant Antibody
Applications
WB IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity Human, Rat
Predicted Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human p16 ARC around N-terminus
Immunogen Range 1-50/151
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 10092
Swiss Prot O15511
Synonyms Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC, ARPC5
Background Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF
Flow-Cyt 1ug/Test
ICC/IF 1:50-100

Frequently asked questions

Which applications is ARPC5 Recombinant Antibody (bsm-52302R) validated for, and at what dilution?

bsm-52302R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF; Flow-Cyt (1ug/Test); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ARPC5 Recombinant Antibody (bsm-52302R) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.

How should bsm-52302R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise ARPC5 Recombinant Antibody (bsm-52302R)?

Recombinant human p16 ARC around N-terminus Immunogen range: 1-50/151.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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