ARPC5 Recombinant Antibody

$Line 1: MCF-7; Line 2: SK-BR-3 lysate probed with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Line 1: MCF-7; Line 2: SK-BR-3 lysate probed with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Flow cytometric analysis of SH-SY-5Y cells with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).

$IF(ICC) staining with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:50 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:50 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bsm-52302r
Product Name	ARPC5 Recombinant Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human p16 ARC around N-terminus
Immunogen Range	1-50/151
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	10092
Swiss Prot	O15511
Synonyms	Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC, ARPC5
Background	Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC