Bax Recombinant Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52316r-fitc-100ul |
| Product Name | Bax Recombinant Antibody, FITC Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | Recombinant human Bak between 20 to 150 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 581 |
| Swiss Prot | Q07812 |
| Subcellular location | Cytoplasm, Mitochondrion, Membrane bound |
| Synonyms | Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BCL2L4 |
| Background | Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | 1:50-200 |
| Flow-Cyt | 1:50-200 |
Frequently asked questions
Which applications is Bax Recombinant Antibody, FITC Conjugated (bsm-52316R-FITC) validated for, and at what dilution?
bsm-52316R-FITC is validated for WB (1:300-5000); IF (1:50-200); Flow-Cyt (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bax Recombinant Antibody, FITC Conjugated (bsm-52316R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52316R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bax Recombinant Antibody, FITC Conjugated (bsm-52316R-FITC)?
Recombinant human Bak between 20 to 150 amino acids
What are the excitation and emission wavelengths of the FITC conjugate (bsm-52316R-FITC)?
FITC on bsm-52316R-FITC: excitation / emission 494nm/518nm.