PCNA Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52347r-100uL |
| Product Name | PCNA Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
FLOW-CYT
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant human PCNA between 100 to 200 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 5111 |
| Swiss Prot | P12004 |
| Synonyms | ProlifeRating cell nuclear antigen, Cyclin, PCNA |
| Background | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF | |
| Flow-Cyt | 1ug/Test |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is PCNA Recombinant Antibody (bsm-52347R) validated for, and at what dilution?
bsm-52347R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF; Flow-Cyt (1ug/Test); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PCNA Recombinant Antibody (bsm-52347R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52347R be stored?
Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise PCNA Recombinant Antibody (bsm-52347R)?
Recombinant human PCNA between 100 to 200 amino acids