PCNA Recombinant Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52347r-apc-Cy5.5 |
| Product Name | PCNA Recombinant Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | Recombinant human PCNA between 100 to 200 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5111 |
| Swiss Prot | P12004 |
| Synonyms | ProlifeRating cell nuclear antigen, Cyclin, PCNA |
| Background | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is PCNA Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-52347R-APC-CY5-5) validated for, and at what dilution?
BSM-52347R-APC-CY5-5 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PCNA Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-52347R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-52347R-APC-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PCNA Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-52347R-APC-CY5-5)?
Recombinant human PCNA between 100 to 200 amino acids
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BSM-52347R-APC-CY5-5)?
APC-Cy5.5 on BSM-52347R-APC-CY5-5: excitation / emission 650nm/694nm.