PTEN Recombinant Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52369r-biotin-100uL |
| Product Name | PTEN Recombinant Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | Human PTEN between 350 amino acids to the C-terminus |
| Clonality | Monoclonal |
| Clone # | 9D2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5728 |
| Swiss Prot | P60484 |
| Synonyms | Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN |
| Background | This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is PTEN Recombinant Antibody, Biotin Conjugated (bsm-52369R-Biotin) validated for, and at what dilution?
bsm-52369R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PTEN Recombinant Antibody, Biotin Conjugated (bsm-52369R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52369R-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PTEN Recombinant Antibody, Biotin Conjugated (bsm-52369R-Biotin)?
Human PTEN between 350 amino acids to the C-terminus