PTEN Recombinant Antibody, PE-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52369r-pe-cy7 |
| Product Name | PTEN Recombinant Antibody, PE-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | Human PTEN between 350 amino acids to the C-terminus |
| Clonality | Monoclonal |
| Clone # | 9D2 |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5728 |
| Swiss Prot | P60484 |
| Synonyms | Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN |
| Background | This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:1000-2000 |
Frequently asked questions
Which applications is PTEN Recombinant Antibody, PE-Cy7 Conjugated (bsm-52369R-PE-Cy7) validated for, and at what dilution?
bsm-52369R-PE-Cy7 is validated for WB (1:1000-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PTEN Recombinant Antibody, PE-Cy7 Conjugated (bsm-52369R-PE-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52369R-PE-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PTEN Recombinant Antibody, PE-Cy7 Conjugated (bsm-52369R-PE-Cy7)?
Human PTEN between 350 amino acids to the C-terminus
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bsm-52369R-PE-Cy7)?
PE-Cy7 on bsm-52369R-PE-Cy7: excitation / emission 488nm/780nm.