Integrin beta 3 (2B11) Monoclonal Antibody

Lane 1: HUVEC cell lysates; Probed with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at a 1:1000 dilution, overnight at 4˚C.

IF(ICC) staining with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue).

IF(ICC) staining with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue).

$Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52374R
Product Name	Integrin beta 3 (2B11) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant Human Integrin beta 3
Immunogen Range	729-788/788
Clonality	Monoclonal
Clone #	2B11
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3690
Swiss Prot	P05106
Subcellular location	Cell membrane, Cytoplasmic domain, Cell junction
Synonyms	Integrin beta-3; Platelet membrane glycoprotein IIIa; CD61; GPIIIa; ITGB3
Background	Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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