CD86 Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52375r-bf594-100uL |
| Product Name | CD86 Recombinant Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | Recombinant human CD86 between 1 to 200 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 942 |
| Swiss Prot | P42081 |
| Synonyms | T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD_antigen: CD86, CD86, CD28LG2 |
| Background | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. |
| Application Dilution | |
| WB | 1:100-200 |
| Flow-Cyt | 1:500-2000 |
Frequently asked questions
Which applications is CD86 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-52375R-BF594) validated for, and at what dilution?
bsm-52375R-BF594 is validated for WB (1:100-200); Flow-Cyt (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD86 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-52375R-BF594) react with?
Tested reactivity: Human.
How should bsm-52375R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD86 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-52375R-BF594)?
Recombinant human CD86 between 1 to 200 amino acids
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-52375R-BF594)?
AbBy Fluor-594 on bsm-52375R-BF594: excitation / emission 590nm/617nm.