N Cadherin Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52389r-apc |
| Product Name | N Cadherin Recombinant Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | Recombinant human N Cadherin between 150 to 250 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1000 |
| Swiss Prot | P19022 |
| Synonyms | Cadherin-2, CDw325, Neural cadherin, CD_antigen: CD325, N-cadherin, CDH2 |
| Background | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is N Cadherin Recombinant Antibody, APC Conjugated (bsm-52389R-APC) validated for, and at what dilution?
bsm-52389R-APC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does N Cadherin Recombinant Antibody, APC Conjugated (bsm-52389R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52389R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise N Cadherin Recombinant Antibody, APC Conjugated (bsm-52389R-APC)?
Recombinant human N Cadherin between 150 to 250 amino acids
What are the excitation and emission wavelengths of the APC conjugate (bsm-52389R-APC)?
APC on bsm-52389R-APC: excitation / emission 650nm/660nm.