NADPH oxidase 4 Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52390r-bf680 |
| Product Name | NADPH oxidase 4 Recombinant Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | Recombinant human NADPH oxidase 4 between 500 to C-terminus |
| Clonality | Monoclonal |
| Clone # | 2A4 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 50507 |
| Swiss Prot | Q9NPH5 |
| Synonyms | NADPH oxidase 4, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase, KOX-1, NOX4, RENOX |
| Background | Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is NADPH oxidase 4 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-52390R-BF680) validated for, and at what dilution?
bsm-52390R-BF680 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NADPH oxidase 4 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-52390R-BF680) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-52390R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NADPH oxidase 4 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-52390R-BF680)?
Recombinant human NADPH oxidase 4 between 500 to C-terminus
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-52390R-BF680)?
AbBy Fluor-680 on bsm-52390R-BF680: excitation / emission 679nm/702nm.