alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52392r-bf488 |
| Product Name | alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Zebrafish |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | Recombinant protein of human alpha smooth muscle Actin between 1-200 amino acids. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 59 |
| Swiss Prot | P62736 |
| Subcellular location | Cytoskeleton |
| Synonyms | Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTA2; ACTSA; alpha smooth muscle Actin |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Application Dilution | |
| WB | 1:2000-10000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:200-800 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-52392R-BF488) validated for, and at what dilution?
bsm-52392R-BF488 is validated for WB(WB) (1:2000-10000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:200-800); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-52392R-BF488) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Zebrafish.
How should bsm-52392R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-52392R-BF488)?
Recombinant protein of human alpha smooth muscle Actin between 1-200 amino acids.
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-52392R-BF488)?
AbBy Fluor-488 on bsm-52392R-BF488: excitation / emission 499nm/519nm.