MKLP1 (7C9) Monoclonal Antibody

$Lane 1: A549 cell lysates; Lane 2: Hela cell lysates probed with \r\nMKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.$

Lane 1: A549 cell lysates; Lane 2: Hela cell lysates probed with \r\nMKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.

$IF(ICC) staining with MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MKLP1 (7C9) Monoclonal Antibody(bsm-52401R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MKLP1 (7C9) Monoclonal Antibody(bsm-52401R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$Lane 1: Rat Lymph node tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates probed with MKLP1 Monoclonal Antibody, Unconjugated (bsm-52401R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Rat Lymph node tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates probed with MKLP1 Monoclonal Antibody, Unconjugated (bsm-52401R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52401R
Product Name	MKLP1 (7C9) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human MKLP1 between 800 to 900 amino acids
Clonality	Monoclonal
Clone #	7C9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9493
Swiss Prot	Q02241
Subcellular location	Nucleus
Synonyms	Kinesin-like protein KIF23, KIF23; Kinesin-like protein 5; Mitotic kinesin-like protein 1; KNSL5; MKLP1
Background	Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200

Applications

Reactivity

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