5T4 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52422r-cy3-100uL |
| Product Name | 5T4 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Recombinant human 5T4 corresponding to aa 80-240 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7162 |
| Swiss Prot | Q13641 |
| Synonyms | Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, M6P1, Wnt-activated inhibitory factor 1, 5T4 oncotrophoblast glycoprotein, WAIF1, TPBG |
| Background | May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | |
Frequently asked questions
Which applications is 5T4 Recombinant Antibody, Cy3 Conjugated (bsm-52422R-Cy3) validated for, and at what dilution?
bsm-52422R-Cy3 is validated for WB(WB) (1:500-2000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 5T4 Recombinant Antibody, Cy3 Conjugated (bsm-52422R-Cy3) react with?
Tested reactivity: Human.
How should bsm-52422R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 5T4 Recombinant Antibody, Cy3 Conjugated (bsm-52422R-Cy3)?
Recombinant human 5T4 corresponding to aa 80-240
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-52422R-Cy3)?
Cy3 on bsm-52422R-Cy3: excitation / emission 512,550nm/570,615nm.