CAMK2 beta Recombinant Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52427R-100uL |
| Product Name | CAMK2 beta Recombinant Antibody |
| Applications |
WB
ELISA
IHC-F
IF
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human CaMKII between 200-300 amino acids |
| Clonality | Recombinant |
| Clone # | 1H12 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 816 |
| Swiss Prot | Q13554 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CAM2; CAMK2; CAMKB; CaMKIIbeta; MRD54; CaMKII; Ck2b; KCC2B_HUMAN; CAMK2B; CaM kinase II subunit beta; CaMK-II subunit beta; 2.7.11.17; KCC2B_MOUSE; Camk2d; KCC2B_RAT; calcium/calmodulin dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; CaM-kinase II beta chain; calcium/calmodulin-dependent protein kinase type II beta chain; CaM kinase II beta subunit; proline rich calmodulin-dependent protein kinase |
| Background | Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. |
| Application Dilution | |
| WB | 1:500-1000 |
| ELISA | 1:5000-10000 |
| IHC-F | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:50-200 |
Frequently asked questions
Which applications is CAMK2 beta Recombinant Antibody (bsm-52427R) validated for, and at what dilution?
bsm-52427R is validated for WB (1:500-1000); ELISA (1:5000-10000); IHC-F (1:50-200); IF (1:50-200); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CAMK2 beta Recombinant Antibody (bsm-52427R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat.
How should bsm-52427R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CAMK2 beta Recombinant Antibody (bsm-52427R)?
Human CaMKII between 200-300 amino acids