PARP Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52433r-bf647-100l |
| Product Name | PARP Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | Human PARP1 between 700-800 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 142 |
| Swiss Prot | P09874 |
| Synonyms | Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, NAD(+) ADP-ribosyltransferase 1, Poly[ADP-ribose] synthase 1, PARP-1, ARTD1, ADPRT 1, PARP1 |
| Background | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | IFICC1:100-500 |
| FCM | WB1:500-2000 |
| IF(IHC-P) | IHC-P1:100-500 |
| IF(ICC) | FCM1:50-100 |
Frequently asked questions
Which applications is PARP Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52433R-BF647) validated for, and at what dilution?
bsm-52433R-BF647 is validated for WB(IF(ICC)) (1:100-500); FCM(WB) (1:500-2000); IF(IHC-P)(IHC-P) (1:100-500); IF(ICC)(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PARP Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52433R-BF647) react with?
Tested reactivity: Human, Mouse.
How should bsm-52433R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PARP Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52433R-BF647)?
Human PARP1 between 700-800 amino acids
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-52433R-BF647)?
AbBy Fluor-647 on bsm-52433R-BF647: excitation / emission 650nm/665nm.