Lactate Dehydrogenase (2D9) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52434r-100uL |
| Product Name | Lactate Dehydrogenase (2D9) Monoclonal Antibody |
| Applications |
WB
IHC-F
IP
IF
IHC-P
FLOW-CYT
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human Lactate Dehydrogenase between 250 to C-terminus |
| Clonality | Monoclonal |
| Clone # | 2D9 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 3939 |
| Swiss Prot | P00338 |
| Synonyms | L-lactate dehydrogenase A chain, Cell prolifeRation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, L-lactate dehydrogenase B chain, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-A, LDH-M, LDHA, PIG19, LDH-B, LDH-H, LDHB |
| Background | Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 8489% sequence similarities and 6975% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-F | 1:100-200 |
| IP | 1:20-50 |
| IF | 1:100-200 |
| IHC-P | 1:100-200 |
| Flow-Cyt | 1:50-100 |
| ICC/IF | 1:50-200 |
Frequently asked questions
Which applications is Lactate Dehydrogenase (2D9) Monoclonal Antibody (bsm-52434R) validated for, and at what dilution?
bsm-52434R is validated for WB (1:500-2000); IHC-F (1:100-200); IP (1:20-50); IF (1:100-200); IHC-P (1:100-200); Flow-Cyt (1:50-100); ICC/IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Lactate Dehydrogenase (2D9) Monoclonal Antibody (bsm-52434R) react with?
Tested reactivity: Human.
How should bsm-52434R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Lactate Dehydrogenase (2D9) Monoclonal Antibody (bsm-52434R)?
Human Lactate Dehydrogenase between 250 to C-terminus