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Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody

Lane 1: Hela cells; Lane 2: NIH\/3T3 cells; Lane 3: SH-SY-5Y cells; Probed with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Hela cells; Lane 2: NIH\/3T3 cells; Lane 3: SH-SY-5Y cells; Probed with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of nih-3t3 cells with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Flow cytometric analysis of nih-3t3 cells with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with Calreticulin (3D1) Monoclonal Antibody (bsm-52435R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Calreticulin (3D1) Monoclonal Antibody (bsm-52435R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Calreticulin (3D1) Monoclonal Antibody (bsm-52435R) at 1:300 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Calreticulin (3D1) Monoclonal Antibody (bsm-52435R) at 1:300 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

  • WB
  • FCM
  • IHC-P
  • IF(ICC)
  • IHC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52435R
Product Name Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody
Applications WB, FCM, IHC-P, IF(ICC), IHC
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic phospho-peptide surrounding S139+S141+S144 of PAK
Modification Site PAK1 (S144)+PAK2 (S141)+PAK3 (S139)
Clonality Monoclonal
Clone # 9A3
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 5063
Swiss Prot O75914
Synonyms Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, p65-PAK, Serine/threonine-protein kinase PAK 2, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, p58, Serine/threonine-protein kinase PAK 3, Beta-PAK, Oligophrenin-3, p21-activated kinase 3, PAK-1, PAK1, PAK-2, PAK2, PAK-3, PAK3
Background Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC

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