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Calreticulin Recombinant Antibody, AbBy Fluor-488 Conjugated

The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green): BF488 conjugated Rabbit Anti-Calreticulin antibody (bsm-52436R-BF488,1:100);Isotype Control (orange): Rabbit IgG\/BF488 (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green): BF488 conjugated Rabbit Anti-Calreticulin antibody (bsm-52436R-BF488,1:100);Isotype Control (orange): Rabbit IgG\/BF488 (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss conjugated antibody and packaging; contents and labels vary by product.
Bioss conjugated antibody and packaging; contents and labels vary by product.

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52436r-bf488
Product Name Calreticulin Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source A synthesized peptide derived from human Calreticulin
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 811
Swiss Prot P27797
Subcellular location Cytoplasm, Secreted, Cell membrane, Extracellular matrix
Synonyms Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA.
Application Dilution
WB 1:2000-10000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:200-800
IF(ICC) IFICC1:100-200

Frequently asked questions

Which applications is Calreticulin Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-52436R-BF488) validated for, and at what dilution?

bsm-52436R-BF488 is validated for WB(WB) (1:2000-10000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:200-800); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Calreticulin Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-52436R-BF488) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-52436R-BF488 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Calreticulin Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-52436R-BF488)?

A synthesized peptide derived from human Calreticulin

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-52436R-BF488)?

AbBy Fluor-488 on bsm-52436R-BF488: excitation / emission 499nm/519nm.

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